Repeated cycles of amplification result in degradation on the probe, leading to amplified fluorescence. TaqMan detection is, therefore, a lot more distinct than dye‐centered detection, simply because an increase in fluorescence occurs only upon binding in the probe to a particular sequence and subsequent de‐quenching, even though no signal is https://www.facebook.com/NewTechFind/posts/pfbid02bYY16kAKZvPhw8EGwH2zkY4jbbAceW1HcTVCraHJztsbpjmNE5g514xZQsFbXtiGl