I've hardly ever tried to freeze the actual biscuits (cooked). I have frozen the raw dough with results. You can give it a shot- my guess is they just could be quite dry soon after defrosting but I am not sure our pups would care, correct? Roll and Slash treats. https://spencerphuhw.actoblog.com/35602804/the-smart-trick-of-healthy-dog-biscuits-that-nobody-is-discussing